Desperation attack:
When fighting as April O'Neil or Casey Jones, watch your life bar. When it begins to blink press Left, Down/Left, Down, Down/Right, Forward + C when close to your opponent.

Easy victory:
Note: This trick requires two controllers. Select one player at the main menu as usual and begin game play. Once in the battle arena and you see your opponent, press Start on controller two. A message stating that a new challenger has arrived will appear. Select the opponent you were about to fight. Proceed to easily defeat player two (your uncontrolled opponent)to complete the level. Note: This trick may only be used up to the first Boss.

Secret rooms:
Begin game play in April or Casey's stage. Slam an enemy twice with April or Casey and they will fall into different room.

Taunts:
Press C to have one of the following characters say their taunt.

April and Clone April: "Come on baby"
Casey and Clone Casey: "So long loser"
Raphael says: "I got you now"
Leonardo says: "Come-on and fight"
Donattello says: "Hey, come and get me"
Michaelangelo says: "That's a laugh"
Sisyphus says: "Crazy punks"
Ray says: "You stink, dirtbag